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KMID : 1025520050470061087
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Journal of Animal Science and Technology
2005 Volume.47 No. 6 p.1087 ~ p.1094
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Molecular Characterization of Hanwoo Glucose Transporter 4 Gene
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Lee Sang-Mi
Jeoung Y. H.
Kim Hye-Min
Pack Hyo-Young
Yoon Du-Hak
Moon Seung-Ju
Chung Eui-Ryong
Kang Man-Jong
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Abstract
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The uptake of glucose for metabolism and growth is essential to most animal cells and is mediated by glucose transport protein. In the glucose transport protein family, GLUT4 plays a key role in cellular glucose uptake stimulated by insulin in skeletal muscles and adipose tissue in rodents and human. In this studies, we reported the identification, characterization, and expression of Hanwoo GLUT4 gene. The Hanwoo GLUT4 cDNA includes a 1527 bp open reading frame encoding a protein of 509 amino acids. The GLUT4 amino acid sequences of the Hanwoo show strong conservation with the corresponding sequences reported in other species. The highest mRNA expression of GLUT4 was detected in heart and lower expression was detected in rib meat, sirloin, and colon. We confirmed the expression of GLUT4 in the subcutaneous and small intestinal adipose tissue using RT-PCR. To investigate the expression of GLUT4 in the bovine intramuscular adipose differentiation, fibroblast-like cells were isolated from the sirloin of Hanwoo bull aged 12 months by collagenase digestion of minced tissue and cultured with activators of PPAR gamma. We identified that GLUT4 mRNA expression decreased during differentiation of preadipocytes into adipocyte in Korean cattle. These results indicated that function of GLUT4 in bovine adipose tissue was different from that of mouse and human.
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KEYWORD
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Adipocyte differentiation, Bovine, Intramuscular preadipocyte
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